Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes

The search for a colorless, nontoxic and efficient drug to prevent transfusion-associated Chagas' disease (TACD) has been underway unsuccessfully since 1953 when gentian violet was preconized and to date is still being used as the only in vitro trypanocidal agent. The recent findings of aminoquinolone "WR6026" as a trypanocidal agent, led the authors to study the metabolism of red cells stored with this compound, the main objective of which was to define its applicability in TACD control. Ten units of human whole blood collected in CPDA-1 were divided into two equal satellite bags. One had "WR6026" (final concentration 62.5æg/mL) added and the other was used as a control, both were stored at 4ºC. At baseline, day 7, 14, 21 and 28, samples were taken for the following measurements: adenosine triphosphate (ATP), hemoglobin, electrolytes (sodium and potassium), gases (pO2 and pCO2) and osmotic fragility. The results of tests and control were analyzed through parametric t-student test. The results were similar in both groups throughout the experiment except for the level of ATP on day 14, which presented significantly higher values in the tests when compared with the controls (p = 0.012). It was concluded that WR6026 does not interfere in the preservation and probably the viability of the erythrocytes also until day 28 of storage. Consequently the authors suggest that WR6026 could emerge as a colorless substitute for gentian violet in the control of TACD in endemic areas

Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes.

The search for a colorless, nontoxic and efficient drug to prevent transfusion-associated Chagas' disease (TACD) has been underway unsuccessfully since 1953 when gentian violet was preconized and to date is still being used as the only in vitro trypanocidal agent. The recent findings of aminoquinolone "WR6026" as a trypanocidal agent, led the authors to study the metabolism of red cells stored with this compound, the main objective of which was to define its applicability in TACD control. Ten units of human whole blood collected in CPDA-1 were divided into two equal satellite bags. One had "WR6026" (final concentration 62.5 g/mL) added and the other was used as a control, both were stored at 4 C. At baseline, day 7, 14, 21 and 28, samples were taken for the following measurements: adenosine triphosphate (ATP), hemoglobin, electrolytes (sodium and potassium), gases (pO2 and pCO2) and osmotic fragility. The results of tests and control were analyzed through parametric t-student test. The results were similar in both groups throughout the experiment except for the level of ATP on day 14, which presented significantly higher values in the tests when compared with the controls (p = 0.012). It was concluded that WR6026 does not interfere in the preservation and probably the viability of the erythrocytes also until day 28 of storage. Consequently the authors suggest that WR6026 could emerge as a colorless substitute for gentian violet in the control of TACD in endemic areas.

A saude do professor do sexo feminino do ensino primario / The female teachers health in elementary school

Neste estudo propos-se:1)averiguar a percentagem de professores com disturbios de saude;2)conhecer as influencias de algumas variaveis(idade,estado civil,grau de escolaridade,tempo de servico,numero de aulas por semana,media de alunos por sala e percentagem de professores que se ausentam frequantemente)aos problemas de saude;3)analisar as principais manifestacoes de morbidade;4)averiguar as cargas relatadas pelas professoras como responsaveis pelo disturbios;5)apontar terapeuticas.A pesquisa foi realizada na rede de ensino estadual,em Uberaba,nos anos de 1991 e 1992numa amostra de 40 professoras selecionadas para serem entrevistadas.Os pricipais resultados foram:75 por cento das professoras relataram alguns disturbios biologico ou psiquico;2)o estado civile o grau de escolaridade foram parametros que apresentaram diferencas estasticamente significante provavelmente relacionadas a alguma morbidade;3)a alteracao mais observada foi a fadiga;4)a carencia de material didatico e o espaco fisico inadequado foram as principais cargas relatadas como responsaveis pelaa alteracoes;5)a curto prazo a terapeutica esta relacionada com a mellhoria salarial que possibilite adequar om orcamento as despesas e aumente a auto-estima do individuo.A medio e a longo prazos a terapeutica pode se vincular a elaboracao de um plano governamental que aumente o investimento nesse setor,valorizando a educacao e a formacao profissional do educador.

Quimioprofilaxia da doença de Chagas transfusional através da associaçäo da violeta de genciana, ascorbato de sódio e luz artificial / Chemoprophylaxis of transfusional Chagas disease by the association of gentian violet, sodium ascorbate and artificial light

A transfusäo de sangue é o segundo mais importante mecanismo de transmissäo da doença de Chagas em países endêmicos. Este trabalho tem como objetivo aprimorar o método de quimioprofilaxia da doença, reduzindo o tempo de esterilizaçäo do sangue chagásico de 24 para 6 horas. Sangue coletado de doadores normais foi infectado com diferentes concentraçöes de duas cepas de T. cruzi de alta e baixa virulência e tratado com a associaçäo de violeta de genciana (VG) 1:4000, ascorbato de sódio (AS) 1:500 e luz durante 6 horas. Em seguida o sangue, lavado ou näo, era inoculado em camundongos, submetido à hemocultura (LIT) e xenodiagnóstico artificial. Os resultados demonstraram que a associaçäo VG+AS e luz durante 6 horas é suficiente para a esterilizaçäo do sangue infectado. Concluimos que, na impossibilidade de se dispor de sorologia segura para doença de Chagas e visando o controle definitivo desta via de transmissäo, a quimioprofilaxia, através do emprego da associaçäo VG, AS e luz, deverá ser seriamente considerada e implementada. Entretanto, säo necessários estudos que permitam reduzir ainda mais o tempo para esterilizaçäo do sangue, favorecendo o rápido emprego deste método quimioprofilático nos pequenos serviços hemoterápicos de regioes endêmicas.